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7.8454 0 0 6.9729 129.3165 486.425 Tm (2)Tj 10.4607 0 0 10.4607 133.6252 488.1826 Tm [(,)-185.1(and)-185.9(0.5)-165(mM)-184.1(PMSF\).)-183.6(Cells)-182.2(were)]TJ -8.7744 -1.1923 TD [(then)-591.4(lysed)-592.3(in)-591.5(nuclear)-589.5(isolation)-591.2(buffer)-592(\(low-salt)]TJ 0 -1.1869 TD [(buffer)-369.8(supplemented)-366.3(with)-363.1(0.5%)-367.1(NP-40,)-370.1(and)-364.8(Com-)]TJ 0 -1.1923 TD [(plete)-261.9(Protease)-262.6(Inhibitor\))-261.7(and)-261.8(passed)-264.4(through)-263(a)-260.9(23-)]TJ T* [(gauge)-346.6(needle)-351.3(three)-345.1(times)-348.6(before)-349.3(centrifugation)-343.1(at)]TJ 0 -1.1869 TD (3,000)Tj /F13 1 Tf 2.9483 0 TD (g)Tj /F5 1 Tf .9972 0 TD [(and)-462.3(4)]TJ /F10 1 Tf 2.7477 0 TD ()Tj /F5 1 Tf .3956 0 TD [(C)-463.5(for)-454.3(5)-168.8(min.)-460.6(Nuclear)-456.5(pellets)-456.6(were)]TJ -7.0889 -1.1923 TD [(washed)-311.3(with)-308.9(nuclear)-307.7(isolation)-309.4(buffer,)-311(and)-310.6(nuclear)]TJ T* [(proteins)-542.2(were)-541.4(extracted)-543.2(with)-541.9(a)-542.8(high-salt)-545.2(buffer)]TJ 0 -1.1869 TD [(\(nuclear)-305.3(isolation)-303.9(buffer)-304.7(supplemented)-306.7(with)-303.5(0.5)-170.4(M)]TJ 0 -1.1923 TD [(NaCl\).)-319.6(Resuspended)-317.1(nuclear)-318.5(extracts)-317.2(were)-319.2(centri-)]TJ T* [(fuged)-307(at)-304.1(16,000)]TJ /F13 1 Tf 7.452 0 TD (g)Tj /F5 1 Tf .8455 0 TD [(and)-305.2(4)]TJ /F10 1 Tf 2.596 0 TD ()Tj /F5 1 Tf .3956 0 TD [(C)-306.3(for)-302.5(5)-163.4(min,)-308.9(and)-305.2(superna-)]TJ -11.2891 -1.1869 TD [(tant)-280.6(was)-279.3(collected)-279.4(as)-279.9(nuclear)-280.6(extract.)]TJ /F2 1 Tf 9.9626 0 0 9.9626 41.8394 332.5038 Tm [(Immu)-10.7(noblot)-337.8(anal)-9.7(ysis)]TJ /F5 1 Tf 10.4607 0 0 10.4607 53.8015 320.0881 Tm -.025 Tc [(Samples)-228.2(w)2.7(ere)-229.5(c)-.7(ombined)-233.4(w)2.7(ith)-229.4(4)]TJ /F10 1 Tf 13.6412 0 TD 0 Tc ()Tj /F5 1 Tf .6883 0 TD -.0253 Tc [(Reducing)-232.2(Sample)]TJ -15.473 -1.1923 TD -.0255 Tc [(Buffer)-386(\(0)-4.6(.25)-167.2(M)-384.6(T)1(ris,)-384(pH)-384.8(6.8,)-384(8%)-384.8(SDS,)-384(40%)-384.8(glycerol,)]TJ T* -.0263 Tc (20%)Tj /F6 1 Tf 2.5472 0 TD 0 Tc (b)Tj /F5 1 Tf .5311 0 TD -.0254 Tc [(-merca)-4.5(ptoethanol,)-687.4(a).9(nd)-689.1(0.01%)-688.2(b)-4.5(r)-1.1(omophenol)]TJ -3.0783 -1.1869 TD [(blue\))-342.7(a)1(nd)-336.7(boiled)-342.1(f)-1.2(or)-342.5(5)-161.6(m)-2(in)-338.1(before)-338.2(separation)-338.1(by)-340.6(elec-)]TJ 0 -1.1923 TD -.0264 Tc [(tro)-3.3(p)-1.8(h)-3.1(o)2.1(re)-3.3(sis)-630.6(o)-3.3(n)-631.8(S)-3(DS)-3(-polya)-5.5(cry)-5.7(l)-1.6(amide)-637.4(g)-.3(e)-3.3(l)-1.6(s.)-634.2(P)-5.3(r)-2.1(ot)-4.5(ein)-3.1(s)]TJ T* -.0247 Tc [(were)-267.2(transf)4.8(erred)-265.6(o)-1.6(nto)-267.2(n)4(itrocellulose)-261.8(membranes)-265.8(\()4.8(GE)]TJ 0 -1.1869 TD -.0253 Tc [(Healthcare\);)-313.4(then,)-313.4(t)2(hey)-308.1(w)-3(ere)-311.1(b)1(locked)-309.6(in)-316.4(TBST)-313.2(\(Tris-)]TJ 0 -1.1923 TD [(HCl,)-497.6(pH)-503.8(7.4,)-503(150)-167(mM)-498.2(NaCl,)-503(a)1(nd)-499.3(0.1%)-503.8(Tween)-500.6(20\))]TJ T* -.0248 Tc [(supplemented)-753.5(w)-2.5(ith)-754.9(5)-3.9(%)-752.6(w)2.9(/v)-752(BSA.)-757.3(Nitrocellulose)]TJ 0 -1.1869 TD -.0241 Tc [(me)4.4(mbrane)4.4(s)-341.1(w)3.6(ere)-337(i).7(ncu)4.6(b)-3.2(ated)-335.5(overn)4.6(i).7(gh)4.6(t)-343.6(w)3.6(ith)-342.3(p).5(rim)4.6(a)2.2(ry)]TJ 0 -1.1923 TD -.0249 Tc [(antibodies)-406.9(at)-404.1(4)]TJ /F10 1 Tf 6.7312 0 TD 0 Tc ()Tj /F5 1 Tf .374 0 TD -.0248 Tc [(C.)-405(Secondar)4.9(y)-410.6(a)1.5(ntibodies)-406.8(c)-.5(onjugated)]TJ -7.1051 -1.1923 TD -.0257 Tc [(wit)-3.8(h)-305.9(ho)-2.6(rseradish)-305.9(p)-1.1(e)-2.6(r)-1.4(oxida)-4.8(s)4.2(e)-306.1(\()-1.6(HRP\))-305.1(w)-3.4(e)2.8(re)-306.1(u)-2.4(s)4.2(e)-2.6(d)-304.6(t)-3.8(o)-300.7(de)-2.6(-)]TJ 0 -1.1869 TD -.0244 Tc [(tect)-409(proteins)-406.4(with)-413(an)-413(e)4.1(nhan)4.3(c)-.1(ed)-411.7(che)4.1(m)-1.1(ilu)4.3(mine)4.1(scen)4.3(ce)]TJ 0 -1.1923 TD -.0248 Tc [(reagent)-328.1(\()-.7(GE)-325.7(Healthcare\).)-329.1(Ni)]TJ 12.6385 0 TD -.0264 Tc [(tro)-3.3(c)-2.1(ellu)-3.1(lose)-333.9(me)-3.3(mbra)-5.5(ne)-3.3(s)]TJ -12.6385 -1.1923 TD -.0252 Tc [(were)-273.1(stripped)-277(i)-.4(n)-272.9(s)-.8(tripping)-275.5(buffer)-277.3(\()-1.1(62.5)-166.9(mM)-275.9(Tris-HCl,)]TJ 0 -1.1869 TD [(pH)-557.9(6)-4.3(.8,)-562.6(2%)-557.9(SDS,)-557.1(and)-558.8(1)-4.3(%)]TJ /F6 1 Tf 12.8228 0 TD 0 Tc (b)Tj /F5 1 Tf .5257 0 TD -.0261 Tc [(-m)-2.8(ercapt)-4.2(oe)-3(th)-2.8(an)-2.8(ol\))-560.2(f)-2(o)-3(r)]TJ -13.3485 -1.1923 TD -.0252 Tc [(10)-166.9(min)-153.7(a)1.1(t)-149.6(6)1.1(0)]TJ /F10 1 Tf 5.2028 0 TD 0 Tc ()Tj /F5 1 Tf .3685 0 TD -.0239 Tc [(C)-151.4(t)3.4(o)-152.6(r).4(em)4.8(ove)-152.6(p).7(re)4.6(viously)-149.5(b)2.4(ou)4.8(nd)-151(antibo)4.6(dies)5.9(.)]TJ /F2 1 Tf 9.9626 0 0 9.9626 41.8394 102.1606 Tm 0 Tc [(Real-t)-8(ime)-336.9(RT-P)-9.4(CR)]TJ /F5 1 Tf 10.4607 0 0 10.4607 53.8015 89.6882 Tm [(qPCR)-668.2(was)-669.5(performed)-669(in)-672.8(quadruplicate)-668.6(with)]TJ -1.1435 -1.1923 TD [(specic)-308.2(primer)-305.5(pairs)-303.8(in)-304.2(MicroAmp)-304.3(Optical)-308(96-well)]TJ 0 -1.1869 TD [(reaction)-529.9(plates)-532.4(with)-531.1(Optical)-535.7(Caps)-529(by)-532.9(using)-533.9(the)]TJ 0 -1.1923 TD [(MyiQ)-578.6(cycler)-584.8(system)-577.5(from)-582.7(Bio-Rad)-576.5(Laboratories)]TJ T* [(\(Hercules,)-271.8(CA\).)-275(In)-269.9(short,)-274.1(the)-273.2(RNeasy)-272.3(Mini)-275.5(Kit)-272.5(and)]TJ 25.4288 64.2875 TD [(the)-305.7(RT2)-307.7(First)-306.3(Strand)-306(Kit)-305(were)-302.9(used)-311.1(to)-301.9(isolate)-308.6(and)]TJ 0 -1.1923 TD [(convert)-462.7(total)-462(RNA)-461.8(into)-459.8(rst-strand)-463.5(cDNA)-461.2(in)-466.8(ac-)]TJ 0 -1.1869 TD [(cordance)-440.9(with)-438.9(the)-441.2(manufacturers)-444.7(protocol.)-440.4(Data)]TJ 0 -1.1923 TD [(were)-622.7(normalized)-620.5(to)]TJ /F6 1 Tf 10.1455 0 TD (b)Tj /F5 1 Tf .5528 0 TD [(-actin)-619.6(mRNA)-622.8(levels)-623.3(as)-621.3(a)]TJ -10.6983 -1.1923 TD [(housekeeping)-407.7(gene)-413.9(and)-413.6(shown)-409.4(as)-409.9(fold)-412.9(change)-411.9(by)]TJ 0 -1.1869 TD [(using)-279.2(the)]TJ /F14 1 Tf 4.5958 0 TD (DD)Tj /F5 1 Tf 1.3332 0 TD [(Ct)-279(method.)]TJ /F2 1 Tf 9.9626 0 0 9.9626 307.8424 631.3888 Tm [(Statis)-8(tical)-339.8(analysi)-8.3(s)]TJ /F5 1 Tf 10.4607 0 0 10.4607 319.8046 618.9164 Tm [(Data)-206.7(are)-208.2(presented)-205(as)-209.4(the)-208.2(mean)]TJ /F10 1 Tf 14.5788 0 TD ()Tj /F5 1 Tf .7208 0 TD [(standard)-205.3(error)]TJ -16.4431 -1.1869 TD [(of)-359.3(the)-359.9(mean)-359.3(of)-359.3(at)-358.3(least)-361.9(three)-361.3(independent)-358.3(experi-)]TJ 0 -1.1923 TD [(ments.)-437.7(Analysis)-441.1(was)-441.9(performed)-435.9(with)-444.4(Sigma)-439.7(Plot)]TJ T* [(version)-400(11.0)-404.3(\(Systat)-400.9(Software,)-397.7(Chicago,)-403.2(IL\),)-400.5(with)]TJ /F13 1 Tf 0 -1.1869 TD (p)Tj /F10 1 Tf .7371 0 TD (<)Tj /F5 1 Tf .7208 0 TD [(0.05)-534.3(considered)-540.9(signicant.)-538.9(Synergy)-537.5(was)-539.4(de-)]TJ -1.4579 -1.1923 TD [(termined)-624.7(by)-625(comparing)-625.4(the)-625.5(combined)-625.7(effect)-629.1(of)]TJ T* [(TLR4)-237.9(ligands)-243(against)-238.8(the)-235.3(predicted)-240(additive)-242.8(effect.)]TJ /F2 1 Tf 10.959 0 0 10.959 307.8424 514.318 Tm (RESULTS)Tj 9.9626 0 0 9.9626 307.8424 500.655 Tm [(FnII)-7.6(I-1c)-337.1(and)-336.9(FnEDA)-340.6(syne)-7(rgisticall)-8.7(y)-335.7(regulat)-8.5(e)]TJ 0 -1.2519 TD [(cytok)-8.9(ine)-265.9(release)-273.6(ups)-7.6(tream)-268.9(of)-270.8(NF-)]TJ /F15 1 Tf 15.6832 0 TD (j)Tj /F2 1 Tf .5577 0 TD [(B)-267.2(activat)-8.1(ion)]TJ /F5 1 Tf 10.4607 0 0 10.4607 319.8046 475.7101 Tm [(P)10.2(r)0(e)20.3(v)0(i)18.4(o)12.2(u)12.4(s)0(l)16.7(y)0(,)-229.4(w)11.4(e)-237.1(h)12.4(av)19.9(e)-237.1(d)0(e)20.6(m)12.4(on)19.2(st)19.2(ra)18.1(t)11(e)12.2(d)-240.9(t)11(h)12.4(a)0(t)-228.2(t)11(w)11.4(o)-237.1(T)10.2(y)0(p)18.2(e)]TJ -1.1435 -1.1869 TD [(II)20.7(I)-472(d)13.8(om)19.3(ai)18.5(n)12.4(s)-468.8(of)-462.3()12.4(b)0(r)18.1(o)12.2(ne)19.2(ct)19.1(in)-456(\(F)18.1(nI)17.4(II)20.7(-)13.2(1)0(c)-458.9(a)0(n)17(d)-468.6(F)10.2(n)12.4(ED)19.5(A\))]TJ 0 -1.1923 TD [(sy)18.1(n)12.4(e)12.2(rg)17.9(is)16.7(t)11(i)13.9(ca)18.1(ll)17(y)-282.8(i)0(n)20.9(d)0(u)20.8(c)0(e)-277.8(t)11(h)12.4(e)-285.8(T)10.2(L)10.2(R)0(4)18.1(-)13.2(de)20.6(pe)15.2(n)12.4(d)0(e)20.6(n)12.4(t)-287(e)12.2(xp)18.2(re)20.3(s-)]TJ T* [(si)22.1(on)-430.6(of)-429.8(in)20.9(a)17(m)12.4(m)12.4(a)0(t)15.7(o)12.2(ry)-431.9(c)13.4(y)0(t)15.5(o)12.2(ki)21.6(ne)19.2(s)-441.7(i)13.9(n)-442.8(h)12.4(um)19.5(an)-432.8(d)13.8(e)0(r)20.2(m)0(a)17.1(l)]TJ 0 -1.1869 TD [()12.4(b)0(r)18.1(o)12.2(bl)18.5(as)18.2(t)11(s)13.6(.)]TJ 7.8454 0 0 6.9729 360.1133 430.5826 Tm [(13)27.3(,1)23.7(5)]TJ 10.4607 0 0 10.4607 386.4188 425.9337 Tm [(In)-676.3(an)-671.2(ef)20.1(fo)20.1(rt)-680.1(t)11(o)-681.5(un)19.4(de)20.6(rs)16.2(t)11(a)0(n)22.5(d)-685.4(th)18.1(e)]TJ -7.5116 -1.1923 TD [(m)12.4(e)0(c)20.3(h)0(a)17(n)12.4(is)16.7(t)11(i)0(c)-471.2(b)0(a)20.1(s)0(i)16.7(s)-479.6(fo)20.1(r)-485.2(t)11(hi)20.9(s)-485(s)0(y)18.1(n)12.4(e)12.2(r)0(g)17.9(y)0(,)-473.3(w)11.4(e)-486.4(e)12.2(xa)19.9(m)12.4(i)0(n)20.9(e)0(d)]TJ T* [(t)11(h)0(r)20.4(e)0(e)-143.5(s)0(t)19.2(e)12.2(ps)-146(o)12.2(f)-154.8(th)18.1(e)-155.8(T)10.2(L)10.2(R)13.5(4-)17.9(NF)20.1(-)]TJ /F6 1 Tf 12.3188 0 TD (j)Tj /F5 1 Tf .542 0 TD [(B)-154.6(p)0(a)18.4(t)11(hw)18.4(ay)-142.7(u)12.4(p)0(s)16.5(t)11(re)20.3(am)-145.5(o)12.2(f)]TJ -12.8607 -1.1869 TD [(IL)20.6(-8)-437.4(pr)21.8(ot)17.9(e)12.2(i)0(n)-439.7(s)13.6(ec)20.2(re)20.3(ti)19.6(on)19.2(:)-445.2(I)0(L)15.2(-)13.2(8)-445.2(m)0(R)20.5(N)0(A)-437.3(l)0(e)20.7(v)0(e)16.7(l)13.9(s,)-437.1(NF)20.1(-)]TJ /F6 1 Tf 21.8844 0 TD (j)Tj /F5 1 Tf .542 0 TD (B)Tj -22.4264 -1.1923 TD [(t)11(r)0(a)18.1(n)12.4(sl)16.7(o)12.2(c)0(a)18.1(t)11(io)20.7(n)-670.5(t)11(o)-665.2(t)0(h)18.1(e)-670.6(n)12.4(u)0(c)20.4(l)0(e)20.7(u)0(s)20.6(,)-672.8(an)22.5(d)-669.1(a)0(c)18.1(t)11.1(iv)18.4(at)21.1(i)13.9(o)0(n)-663.6(o)12.2(f)]TJ T* [(t)11(h)0(e)-533.6(u)12.4(ps)22(tr)19.1(ea)16.9(m)-534.9(k)0(i)16.2(n)12.4(as)18.2(e)12.2(,)-542.8(IK)21.8(K)11.4(.)-542.8(Q)11.4(u)12.4(an)17(t)11(i)13.9(ta)15.7(t)11(i)0(v)18.4(e)-535.1(re)20.3(ve)16.7(r)13.4(s)0(e)]TJ 0 -1.1869 TD [(t)11(r)0(a)18.1(n)12.4(sc)16.2(r)13.4(i)0(p)16.9(t)11(io)20.7(n-)20.3(po)20.6(ly)18.4(m)12.4(e)0(r)20.2(a)0(s)18.2(e)-193.7(ch)20.4(ai)18.5(n)-193.5(r)0(e)20.3(a)0(c)18.1(t)11(io)20.7(n)-198.9(\()13.2(qR)18.1(T)10.2(-)0(P)18.1(C)13.5(R\))]TJ 0 -1.1923 TD [(an)22.5(al)18.5(ys)18.1(i)13.9(s)-539.2(in)20.9(di)16.9(ca)18.1(t)11(e)12.2(d)-539(t)11(ha)17(t)-536.3(i)0(n)20.9(d)0(i)16.9(v)0(i)23.8(d)0(u)15.4(a)0(l)24(l)0(y)-529(F)10.2(nE)20.5(DA)-527.9(an)17.1(d)]TJ T* [(F)10.2(n)0(I)17.4(I)0(I)20.7(-)13.2(1c)-361.3(in)20.9(du)15.4(c)13.4(e)0(d)-364.2(a)-369.3(6-)-361.5(an)17(d)-371(2)0(4)20.1(-)13.2(fo)20.1(ld)-362.5(in)20.9(cr)16(e)12.2(a)0(s)23.6(e)-372.6(in)-363.9(IL)20.6(-8)]TJ 0 -1.1869 TD [(m)12.4(R)0(N)17.9(A)0(,)-339.6(r)0(e)20.3(s)0(p)22(e)0(c)20.2(t)0(i)19.6(v)0(e)16.7(l)13.9(y;)-343.2(h)12.4(o)0(w)18.2(e)12.2(ve)22.1(r,)-339.7(wh)18.4(e)12.2(n)-350.7(ce)20.3(ll)17(s)-349.5(w)11.4(e)12.2(r)0(e)-342.9(i)13.9(n-)]TJ 0 -1.1923 TD [(cu)20.4(ba)20.1(t)11(e)0(d)-136.6(w)11.4(it)19.6(h)-150.2(b)0(o)22.3(t)11(h)-150.2(d)13.8(om)19.3(ai)18.5(n)12.4(s)0(,)-139(I)0(L)20.6(-)13.2(8)-147.1(m)0(R)20.5(N)0(A)-139.2(w)11.4(as)-133.5(in)20.9(du)15.4(c)13.4(e)0(d)]TJ T* [(by)-359.4(36)20.1(6-)23.3(fo)20.1(ld)-362.5(\()13.2(F)10.2(ig)18.4(.)-369.4(1)0(A)18.1(\))13.2(.)-369.4(T)0(h)17.2(e)12.2(se)-359(n)19.4(di)16.9(n)12.4(g)0(s)-361.3(i)0(n)20.9(d)0(i)22.3(c)0(a)18.1(t)11(e)-372.6(t)11(h)12.4(a)0(t)]TJ 0 -1.1869 TD [(t)11(h)0(e)-457.7(c)0(o)20.3(m)12.4(bi)18.5(n)12.4(a)0(t)15.7(i)13.9(on)-457.7(o)12.2(f)-469.1(t)11(h)0(e)-457.7(t)11(w)11.4(o)-470.1(d)13.8(o)0(m)19.3(a)0(i)18.5(n)12.4(s)-468.8(r)13.4(es)20.4(ul)15.5(t)11(s)-463.3(in)-461.4(a)]TJ 0 -1.1923 TD [(sy)18.1(n)12.4(e)12.2(rg)17.9(is)16.7(t)11(i)13.9(c)-306.3(i)0(n)20.9(c)0(r)16(e)12.2(as)18.2(e)-302.1(\()]TJ /F13 1 Tf 9.609 0 TD -.0156 Tc [(i.)-15.6(e.)]TJ /F5 1 Tf 1.3007 0 TD 0 Tc [(,)-304.3(s)0(i)16.7(g)15.3(ni)15.5()12.4(c)0(a)18.1(n)12.4(t)11(l)0(y)-296(g)0(r)17.9(e)12.2(at)21.1(er)-294.1(th)18.1(an)]TJ -10.9097 -1.1923 TD [(t)11(h)0(e)-197.5(p)0(r)16.4(e)12.2(di)16.9(c)13.4(t)0(e)17.9(d)-208.4(ad)18.4(di)22.3(ti)19.6(ve)-200.1(in)20.9(cr)16(e)12.2(a)0(s)23.6(e)-210(sh)20.6(ow)18.2(n)-209.8(i)13.9(n)-209.8(t)11(he)-203(d)13.8(a)0(s)18.2(h)12.4(ed)]TJ 0 -1.1869 TD [(li)17(n)12.4(e)-269.6(b)15.5(a)0(r)18.1(s)0(\))-260.4(i)13.9(n)-269.4(m)12.4(RN)17.9(A)-268.4(p)13.8(ro)14.8(d)13.8(u)0(c)20.4(t)0(i)19.6(o)0(n)19.2(.)-266.4(In)-259(ad)18.4(d)13.8(i)0(t)19.6(i)0(o)20.7(n)-269.4(t)11.1(o)-269.6(IL)20.6(-8)23.3(,)]TJ 0 -1.1923 TD [(w)11.4(e)-318.4(h)12.4(a)0(v)19.9(e)-312.9(id)16.9(e)12.2(n)0(t)18.1(i)0()20.9(e)12.2(d)-316.8(s)0(e)20.4(v)0(e)22.1(r)0(a)18.1(l)-316.7(pr)21.8(o-)20.1(in)20.9(a)17.1(m)12.4(m)0(a)22.5(t)0(o)17.9(r)0(y)-307.3(g)0(e)22.1(n)12.4(es)]TJ T* [(t)11(h)0(a)17(t)-352.1(ar)18.1(e)-356.3(u)0(p)20.8(r)0(e)20.2(g)0(u)16.9(l)0(a)24(t)0(e)17.9(d)-354.8(in)-347.6(hu)19.4(m)12.4(a)0(n)-351.5(d)13.8(er)20.3(ma)17.1(l)-354.6()12.4(br)18.1(o)12.2(b)0(l)18.6(a)0(s)18.2(t)11.1(s)]TJ 0 -1.1869 TD [(af)17.9(t)11(e)12.2(r)-268.4(F)10.2(nI)17.4(II)20.7(-1)17.9(c)-268.4(a)0(n)22.5(d)-273.5(F)10.2(n)12.4(ED)19.5(A)-268.4(t)11(re)20.3(at)15.7(m)12.4(e)12.2(nt)18.1(,)-271.8(i)13.9(nc)20.4(lu)15.5(d)13.8(i)0(n)15.5(g)-266.5(t)11(u)0(-)]TJ 0 -1.1923 TD [(m)12.4(o)0(r)-234.5(n)12.4(ec)20.3(ro)20.3(si)16.7(s)-241.1(f)0(a)23.3(c)0(t)19.1(o)0(r)20.3(-)0(a)17.9(l)0(p)22.3(h)0(a)-232.3(\()0(T)18.1(N)0(F)20.1(-)]TJ /F6 1 Tf 14.4487 0 TD (a)Tj /F5 1 Tf .4932 0 TD (\))Tj 7.8454 0 0 6.9729 467.4896 193.9464 Tm [(13)27.3(,1)23.7(5)]TJ 10.4607 0 0 10.4607 489.1463 189.2976 Tm [(an)22.5(d)-240.9(v)0(a)19.9(s)0(c)16.2(u)12.4(la)18.5(r)]TJ -17.3319 -1.1923 TD [(ce)20.3(ll)-530.4(ad)23.8(he)19.2(si)22.1(on)-528.1(mo)19.3(le)20.7(cu)20.4(le)20.7(-1)-529.5(\()13.2(V)0(C)16.1(A)13.5(M-)17.2(1\))-524.1(\(u)20.2(np)20.8(ub)17(li)22.4(sh)20.6(ed)]TJ 0 -1.1869 TD [(o)12.2(b)0(s)18.2(e)12.2(rv)17.9(at)21.1(io)20.7(ns)20.6(\).)-253.1(A)-263(s)0(y)18.1(n)12.4(er)20.2(gi)18.4(st)19.2(ic)-254.5(i)13.9(n)0(c)15(r)13.4(ea)22.3(se)-256(in)-250.1(bo)16.9(t)11(h)-264(T)10.2(N)15.3(F-)]TJ /F6 1 Tf 22.6431 0 TD (a)Tj /F5 1 Tf -22.6431 -1.1923 TD 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7 0 obj <>stream PGOHBE+AdvPSMP12 yMM AdvPSMP12Kl0xwD m>SxţyU?llU7}oa@P]WhbB:cfw endstream endobj 8 0 obj <> endobj 9 0 obj <> endobj 10 0 obj <>/Font<>/ProcSet[/PDF/Text/ImageB]/ExtGState<>>> endobj 11 0 obj <>stream /GS1 gs BT /F5 1 Tf 10.4607 0 0 10.4607 328.0251 712.3463 Tm 0 0 0 rg 0 Tc 0 Tw [(w)11.4(h)0(e)19.2(r)0(e)20.3(a)0(s)-220.2(b)0(o)22.3(t)0(h)-225.8()12.4(br)18.1(o)12.2(n)12.4(ec)14.8(t)11(i)13.9(n)-236.9(d)13.8(om)19.3(ai)18.5(n)12.4(s)-230.3(in)15.5(d)13.8(u)0(c)20.4(e)0(d)-223.3(a)-233.8(54)25.5(-f)15.7(o)12.2(l)0(d)]TJ 0 -1.1923 TD [(ch)20.4(an)17(ge)-324.7(w)11.4(h)0(e)19.2(n)-334.4(co)20.3(mb)17.1(i)13.9(n)0(e)19.2(d)-338.5(\(F)18.1(i)13.9(g)0(.)-327(1)0(C)18.1(\))0(.)-329(T)10.2(h)12.4(e)12.2(se)-326.4(da)18.4(t)11(a)-336.8(in)20.9(di)16.9(-)]TJ 0 -1.1869 TD -.012 Tc 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[(\(Fig.)-365.4(2A\).)-365.1(The)-366.1(data)-367(for)-367.6(the)-365.3(60-min)-366.9(time)-362.2(point)-370.1(are)]TJ 0 -1.1923 TD [(quantied)-395.6(in)-396.4(Fig.)-400.3(2B)-393.8(and)-397.3(show)-394.7(that)-394.4(FnEDA)-399.7(and)]TJ T* [(FnIII-1c)-223.8(individually)-222.1(stimulated)-222.4(a)-217.6(1.8-)-222.2(and)-223.9(2.6-fold)]TJ 0 -1.1869 TD [(increase)-543.6(in)-548.1(NF-)]TJ /F6 1 Tf 7.6579 0 TD (j)Tj /F5 1 Tf .5528 0 TD [(B-p65)-546.4(translocation)-544.7(to)-545.8(the)-544.2(nu-)]TJ -8.2107 -1.1923 TD [(cleus,)-325.4(respectively.)-322.7(When)-322.5(added)-326.6(together,)-323.6(the)-322(two)]TJ T* [(domains)-350.5(stimulated)-357.8(a)-353.1(9.3-fold)-347.9(increase)-353.9(in)-358.5(the)-349.1(nu-)]TJ 0 -1.1869 TD [(clear)-446.4(localization)-446.1(of)-451.4(NF-)]TJ /F6 1 Tf 11.5221 0 TD (j)Tj /F5 1 Tf .5474 0 TD [(B-p65,)-449.7(which)-445.8(is)-449.4(signi-)]TJ -12.0695 -1.1923 TD [(cantly)-308.6(greater)-307.9(than)-311.7(the)-305.7(expected)-313.2(additive)-307.8(effect)-309.3(of)]TJ T* [(4.5-fold)-304.5(\(dashed)-301.1(line)-305.2(bar\).)-300.9(A)-300.9(similar)-304.3(synergy)-303.4(could)]TJ 0 -1.1869 TD [(also)-421.7(be)-416.7(seen)-415.6(at)-423.3(the)-419.5(120)-165(min)-416.4(time)-421.8(point)-418.9(\(data)-418.8(not)]TJ 0 -1.1923 TD (shown\).)Tj 1.1435 -1.1923 TD [(Activation)-436.8(of)-435.2(the)-430.4(TLR4-NF-)]TJ /F6 1 Tf 13.148 0 TD (j)Tj /F5 1 Tf .5528 0 TD [(B)-436.4(signaling)-431.9(path-)]TJ -14.8443 -1.1869 TD [(way)-467.3(initially)-472.3(results)-467.9(in)-472.3(the)-468.3(homodimerization)-470.3(of)]TJ 0 -1.1923 TD [(TLR4)-194.5(receptors,)-194.3(ultimately)-193.4(leading)-195.6(to)-193.5(the)-191.9(assembly)]TJ T* [(of)-326.8(a)-331.4(scaffold)-324.6(of)-332.2(associated)-325.9(kinases,)-329.2(which)-326.6(regulate)]TJ 0 -1.1869 TD [(the)-370.8(phosphorylation)-376.2(of)-370.2(IKK.)-374.1(Once)-367.8(activated,)-372.3(IKK)]TJ 0 -1.1923 TD [(phosphorylates)-340.2(the)-343.7(NF-)]TJ /F6 1 Tf 11.0181 0 TD (j)Tj /F5 1 Tf .5528 0 TD [(B)-344.2(inhibitor,)-342.7(I)]TJ /F6 1 Tf 6.1404 0 TD (j)Tj /F5 1 Tf .5528 0 TD [(B,)-345.1(thereby)]TJ -18.2641 -1.1923 TD [(releasing)-338.1(the)-332.8(transcriptionally)-340.2(active)-335.9(p65)-340.1(subunit)]TJ 0 -1.1869 TD [(to)-464.5(enter)-464.3(the)-468.3(nucleus.)-463.1(To)-465.3(determine)-460.7(whether)-467.6(the)]TJ 0 -1.1923 TD [(phosphorylation)-528(of)-521.9(IKK)-525(reected)-527.8(the)-527.9(synergism)]TJ T* [(observed)-319.8(at)-320.4(the)-322(level)-317.2(of)-321.4(NF-)]TJ /F6 1 Tf 12.9149 0 TD (j)Tj /F5 1 Tf .5528 0 TD [(B)-322.6(translocation,)-317.9(IL-8)]TJ -13.4677 -1.1869 TD [(transcription,)-551.4(and)-549.1(protein)-545(expression,)-550.9(broblast)]TJ 0 -1.1923 TD [(lysates)-219.2(were)-221.6(collected)-219.7(after)-216.4(the)-219(addition)-224(of)-218.4(FnIII-1c)]TJ T* [(and)-424.4(FnEDA,)-427.6(alone)-421.5(and)-424.4(in)-423.5(combination.)-424.8(Cells)-426(in-)]TJ 0 -1.1869 TD [(cubated)-816.6(with)-812.9(the)-815.2(control)-816.3(domain)-813.9(\(FnIII-10n\))]TJ 0 -1.1923 TD [(showed)-249.5(no)-251.7(activation)-250.5(of)-250.9(IKK)-248.6(over)-250.3(the)-251.5(120)-165(min)-248.4(time)]TJ T* [(course)-204.4(\(Fig.)-202.8(3A\).)-202.5(The)-203.6(FnEDA)-204.6(and)-202.2(FnIII-1c)-202.2(domains)]TJ 0 -1.1869 TD [(showed)-292.8(modest)-293.2(increases)-291.5(in)-293.4(IKK)-292(phosphorylation;)]TJ 0 -1.1923 TD [(however,)-561.9(there)-556.4(was)-561.1(a)-559(marked)-559(increase)-559.8(in)-559(IKK)]TJ T* [(phosphorylation)-528(when)-531.4(both)-528.7(domains)-529.4(were)-530.6(incu-)]TJ 0 -1.1869 TD [(bated)-219.2(with)-216.7(cells.)-220.9(Quantication)-217.1(of)-218.4(data)-215.2(obtained)-218.6(by)]TJ 0 -1.1923 TD [(image)-217.9(analysis)-211.5(of)-218.4(Western)-214.2(blots)-215.5(representing)-217.8(three)]TJ 0 -1.1869 TD [(different)-372.2(experiments)-372.2(demonstrated)-371.9(that)-367.3(synergy)]TJ 0 -1.1923 TD [(between)-314(the)-316.6(two)-312.1(modules)-315.8(is)-313.9(evident)-315.5(at)-314.9(the)-316.6(60)-315.9(and)]TJ T* [(120)-165(min)-275.5(time)-280.9(points)-275.3(\(Fig.)-278.7(3B\).)-278.4(Densitometric)-275.5(units)]TJ 0 -1.1869 TD [(representing)-602.6(p-IKK)-600.9(indicate)-603.7(that)-605.8(incubation)-604.3(of)]TJ 0 -1.1923 TD [(dermal)-433.6(broblasts)-437.8(with)-433.5(FnEDA)-432.2(and)-435.2(FnIII-1c)-435.2(for)]TJ T* [(60)-164.2(min)-226.8(resulted)-227.9(in)-228.4(densitometric)-229.8(units)-223.8(of)-229.2(0.086)-226.2(and)]TJ 0 -1.1869 TD [(0.045,)-275.8(respectively;)-273.9(however,)-269.2(incubation)-273.7(with)-276.4(both)]TJ 0 -1.1923 TD [(FnEDA)-318.4(and)-316(FnIII-1c)-316(for)-313.4(60)-169.6(min)-313.5(resulted)-314.6(in)-320.5(densi-)]TJ T* [(tometric)-214.5(units)-218.4(of)-218.4(0.98,)-215.4(consistent)-215.4(with)-216.7(a)-217.6(synergistic)]TJ ET 62.022 49.72 242.135 .51022 re f q 1 i 64.12 719.886 m 302.06 719.886 l 302.06 200.239 l 64.12 200.239 l W n q 238.023 0 0 519.647 64.12 200.239 cm /Im3 Do Q Q BT /F1 1 Tf 7.9702 0 0 7.9702 62.022 176.2582 Tm [(Figure)-295.4(1.)]TJ /F16 1 Tf 4.6235 0 TD [(FnIII-1c)-294.5(and)-299.6(FnEDA)-291.2(modules)-294.5(stimulate)-291.9(a)-303.8(synergistic)-289.5(increase)-293.3(in)]TJ -4.6235 -1.2519 TD [(the)-241.2(expression)-231.6(of)-243.3(bro-inamm)13.6(atory)-243.7(genes.)-237.2(Conuent)-235.7(monolaye)13.4(rs)-244.3(of)-243.3(human)]TJ 0 -1.2448 TD [(dermal)-353.7(broblas)12.5(ts)-360.3(were)-353.2(serum)-354.4(starved)-349.4(overnigh)12.5(t;)-361.7(then,)-348.3(they)-356.2(were)-353.2(treated)]TJ 0 -1.2519 TD [(with)-338.7(2)]TJ /F6 1 Tf 2.6674 0 TD (l)Tj /F16 1 Tf .5477 0 TD [(M)-340.7(each)-333.1(of)-335.8(FnIII)-336.8(domains,)-332.6(alone)-328.5(and)-335.2(in)-341.9(combination)12.7(.)-336.8(Data)-338.5(are)-331.5(pre-)]TJ -3.2151 -1.2519 TD [(sented)-231.1(as)-240.9(fold)-236.6(increase)-229.3(over)-240.1(the)-234.1(control)-233.8(module,)-231.9(FnIII-10n,)-234.1(which)-231.4(was)-234.2(set)-237.3(at)]TJ T* [(1.)-252.6(The)-249.8(theoretical)-242.8(expected)-241.1(additive)-250.3(effect)-246.2(of)-250.4(combining)-241.9(FnEDA)-248.5(and)-249.8(FnIII-1c)]TJ 0 -1.2448 TD [(is)-345.1(represen)13.7(ted)-340.8(by)-340.5(the)]TJ /F17 1 Tf 9.1404 0 TD [(open)-340.2(dashed)-332.2(line)]TJ /F16 1 Tf 7.3977 0 TD [(bar.)-334(After)-338.3(a)-346.5(2)-164.7(h)-339.4(incubation)-335.4(period,)]TJ -16.538 -1.2519 TD [(mRNA)-323.7(was)-333.8(isolated)-321.5(and)-328.1(analyzed)-319.6(via)-335.9(qRT-PCR)-321.5(by)-326.3(using)-324.6(primers)-329.8(to)-328.7(human)]TJ T* (IL-8)Tj /F1 1 Tf 2.0344 0 TD (\(A\))Tj /F16 1 Tf 1.3799 0 TD [(,)-528.9(TNF-)]TJ /F6 1 Tf 2.6888 0 TD (a)Tj /F1 1 Tf 1.0314 0 TD (\(B\))Tj /F16 1 Tf 1.3302 0 TD [(,)-528.9(and)-520.1(human)-525.6(VCAM-1)]TJ /F1 1 Tf 9.8588 0 TD (\(C\))Tj /F16 1 Tf 1.3302 0 TD [(.)-528.9(Statistical)-520.9(analysis)-521.4(was)]TJ -19.6536 -1.2448 TD [(performed)-259.4(by)-269.3(using)-267.7(a)-268.2(Students)]TJ /F17 1 Tf 12.8819 0 TD (t)Tj /F16 1 Tf .2774 0 TD [(-test.)-266(**)]TJ /F17 1 Tf 3.2222 0 TD (p)Tj /F10 1 Tf .6615 0 TD (<)Tj /F16 1 Tf .7184 0 TD [(0.01.)-271.6(Data)-260.3(are)-267.5(presented)-263.8(as)-262.2(the)]TJ -17.7615 -1.2519 TD (mean)Tj /F10 1 Tf 2.3758 0 TD ()Tj /F16 1 Tf .7184 0 TD [(S.E.M.)-448.5(\()]TJ /F17 1 Tf 3.2649 0 TD (n)Tj /F10 1 Tf .6615 0 TD (=)Tj /F16 1 Tf .7184 0 TD [(3\).)-448.2(EDA,)-444(extra)-449.9(domain)-435.6(A;)-448.4(IL-8,)-450.6(interleuk)11.5(in)-448.6(8;)-451.8(qRT-PCR,)]TJ -7.7391 -1.2519 TD [(quantitative)-423.9(reverse)-432.6(transcription)10.9(-polymerase)-424.2(chain)-428.1(reaction;)-433.4(SEM,)-429.3(stan-)]TJ T* [(dard)-329.4(error)-327(of)-335.8(the)-326.6(mean.)]TJ /F11 1 Tf 8.9663 0 0 8.9663 62.022 758.6644 Tm (312)Tj /F12 1 Tf 9.9626 0 0 9.9626 277.7952 758.6644 Tm 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[(-test.)-330(*)]TJ /F17 1 Tf 2.8381 0 TD (p)Tj /F10 1 Tf .6686 0 TD (<)Tj /F16 1 Tf .7113 0 TD [(0.05.)-335.6(Data)-331.4(are)-324.4(presented)-327.8(as)-326.3(the)-333.7(mean)]TJ /F10 1 Tf 15.6489 0 TD ()Tj /F16 1 Tf .7184 0 TD [(S.E.M.)-327.6(\()]TJ /F17 1 Tf 3.1511 0 TD (n)Tj /F10 1 Tf .6615 0 TD (=)Tj /F16 1 Tf .7184 0 TD [(3\).)-327.3(NF-)]TJ /F6 1 Tf 2.7172 0 TD (j)Tj /F16 1 Tf .5548 0 TD [(B,)-334.6(nuclear)-320.6(factor)-329.3(kappa-ligh)12.1(t)-337.8(chain)-321.4(enhancer)-328.2(of)-328.7(activated)-327.5(B)-332.1(cells.)]TJ /F12 1 Tf 9.9626 0 0 9.9626 163.559 757.8707 Tm [(INDUCT)-11.7(ION)-336.9(OF)-334.3(IL-8)-337.1(BY)-336.1(FIBR)-8.8(ONECT)-9.3(IN)-332.6(TYPE)-343.1(III)-334.9(DOMAI)-9.9(NS)]TJ /F11 1 Tf 8.9663 0 0 8.9663 535.0676 757.8707 Tm (313)Tj ET 41.839 753.959 508.082 .51025 re f endstream endobj 16 0 obj <>stream AdobedC        s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjz?Nlٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͛6lٳf͌bHhB1 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Kw$H>IO-N&hW1Ϭj635˵e@ſϗu(]-'MjPp9_ZK8C׳fyEޱo޽"3*$ Jo,ny\h:ac\6-pJEIL` W -2y#N_ҒKuČo,ӻOMHȏ|8Ə}?)>Ko3t4D$[G,a"*3f͛6lٳf͛6lٳf͛6lٳS躿yBMY Gi ʷo0j٨mt*op^ tD4_ y=0Ma]_Fx߳)Xvoo}E4Z9lbM2E9+J,j6Jԯ5[2h w]&:Kfŭ5-"\+OkFX=FXhUX&J:DW3NtY9 C;8W#~x^h֧ũyzPK_DoG Iہ^C<ow7FRZTW MI2?l_4,v> endobj 27 0 obj <>/Font<>/ProcSet[/PDF/Text/ImageC]/ExtGState<>>> endobj 28 0 obj <>stream /GS1 gs BT /F5 1 Tf 10.4607 0 0 10.4607 73.9842 712.3463 Tm 0 0 0 rg 0 Tc 0 Tw [(The)-279.4(myddosome)-277.5(is)-281.4(a)-282.6(signaling)-280.2(platform)-284.6(that)-280.6(as-)]TJ -1.1435 -1.1923 TD [(sembles)-215.4(on)-213.8(the)-213.6(cytoplasmic)-214.5(tail)-211.2(of)-218.4(the)-213.6(TLR)-215.4(receptor)]TJ 0 -1.1869 TD [(and)-424.4(consists)-421.8(of)-424.3(the)-425(adaptor)-419.6(proteins)-423(MyD88)-426.1(and)]TJ 0 -1.1923 TD [(TIRAP,)-284.5(which)-283.2(initiate)-280.8(signaling)-280.2(through)-284.6(the)-284(bind-)]TJ T* [(ing)-500.3(of)-505.6(IL-1)-498.1(receptor-associated)-503.4(kinases)-496.4(\(IRAKs\),)]TJ 0 -1.1869 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[(associated)-331.3(proteins)-330.9(that)-334.8(are)-332.8(involved)-335.3(in)-331.3(transmit-)]TJ T* [(ting)-532.6(LPS/TLR4-dependent)-533.6(signals,)-531.3(although)-534.2(the)]TJ 0 -1.1869 TD [(interrelationship)-298.8(among)-300.6(these)-296.1(proteins)-303.8(is)-297.7(not)-300.3(well)]TJ 0 -1.1923 TD (understood.)Tj 7.8454 0 0 6.9729 384.9448 642.2738 Tm (28,33,34)Tj 10.4607 0 0 10.4607 420.2078 637.625 Tm [(Our)-461.3(studies)-462.2(show)-465.2(that)-464.9(inhibi-)]TJ -8.8123 -1.1923 TD [(tors)-323.6(of)-326.8(EGFR)-325.4(and)-321.4(Src)-329.1(kinases)-323(attenuated)-322.6(IL-8)-324.6(re-)]TJ 0 -1.1869 TD [(lease)-452.8(in)-450.6(response)-454.7(to)-448.2(the)-452(matrix-derived)-455.3(DAMPs,)]TJ 0 -1.1923 TD [(FnEDA)-616.5(and)-614.1(FnIII-1c.)-614.9(The)-615.5(inhibition,)-617(although)]TJ T* [(signicant,)-457.6(was)-452.7(only)-455.6(partial)-455.7(\()]TJ /F14 1 Tf 14.1777 0 TD (*)Tj /F5 1 Tf .9972 0 TD [(50%\),)-451.4(suggesting)]TJ -15.1749 -1.1869 TD [(that)-448.6(some)-448.1(of)-451.4(the)-452.1(signaling)-448.2(downstream)-451.8(of)-446(TLR4)]TJ 0 -1.1923 TD [(was)-279.3(independent)-277(of)-283.4(EGFR)-276.6(and)-278.1(Src)-280.3(activity.)]TJ 1.1435 -1.1923 TD [(Our)-553.5(data)-551.2(indicate)-554.9(that)-557(individual)-554.8(bronectin)]TJ -1.1435 -1.1869 TD [(Type)-441.6(III)-445.8(domains)-442.6(can)-441(synergize)-442.2(with)-444.4(each)-439.6(other)]TJ 0 -1.1923 TD [(and)-196.8(with)-195.1(LPS)-194.6(to)-193.5(regulate)-192(IL-8)-194.6(expression)-197.8(in)-195.9(human)]TJ ET 62.022 39.742 508.082 .51025 re f q 1 i 154.035 498.841 m 478.148 498.841 l 478.148 140.428 l 154.035 140.428 l W n q 324.113 0 0 358.412 154.035 140.428 cm /Im8 Do Q Q BT /F1 1 Tf 7.9702 0 0 7.9702 62.022 116.4472 Tm [(Figure)-288.3(6.)]TJ /F16 1 Tf 4.6164 0 TD [(Regulation)-283.4(of)-300.2(IL-8)-291.6(expression)-288.5(by)-290.7(LPS)-291.3(and)-292.5(FnIII)-294.2(domains.)-289.9(The)-292.5(model)-288.3(depicts)-290.5(the)-291(activation)-287.3(of)-293.1(TLR4)-294.7(signaling)-289.9(in)-292.1(response)-284.8(to)-293.1(LPS)-298.4(and)-285.4(FnIII)-294.2(domains.)]TJ -4.6164 -1.2448 TD [(LPS)-262.9(binds)-260.6(to)-264.6(both)-267.6(CD14,)-261.4(a)-261.1(GPI-linked)-261.4(protein,)-257.6(and)-264.1(MD2,)-263.8(a)-268.2(TLR4-asso)11.8(ciated)-260.9(protein.)-264.7(The)-257(interaction)-257(of)-264.6(LPS)-262.9(with)-267.6(these)-261.6(molecule)12.1(s)-271.4(triggers)-259.6(TLR4)-259.1(dimerizat)]TJ 60.7786 0 TD [(ion)-257.6(and)]TJ -60.7786 -1.2519 TD [(activation)-266(of)-278.9(downstream)-266.7(signaling)-268.6(to)-271.7(NF-)]TJ /F6 1 Tf 16.9008 0 TD (j)Tj /F16 1 Tf .5548 0 TD [(B)-275.1(and)-271.2(mitogen-act)15.4(ive)-279(protein)-269.3(kinase)-268.8(pathways)-268.2(such)-272.3(as)-276.5(ERK.)-276.4(Activation)-265.8(of)-271.8(the)-276.8(NF-)]TJ /F6 1 Tf 32.9836 0 TD (j)Tj /F16 1 Tf .5477 0 TD [(B)-275.1(and)-278.3(AP1)-272(transcription)-266.6(factors)]TJ -50.9869 -1.2519 TD [(then)-303.2(induces)-299.1(the)-305.2(expression)-302.7(of)-307.3(IL-8)-305.9(mRNA)-302.4(and)-306.7(protein)-297.8(synthesis.)-305.6(EGFR)-305.5(and)-299.6(Src)-307.9(kinases)-305.5(have)-300.8(been)-304.7(demonstrat)14.7(ed)-308.8(to)-307.3(regulate)-302.8(the)-305.2(TLR4-d)12(ependent)-302(release)-302.5(of)]TJ T* [(cytokines)-271.4(by)-276.5(LPS.)-279.6(The)-271.2(current)-275.3(study)-271.9(has)-281.5(now)-273.7(implicated)-271.4(Src)-272.4(and)-278.3(EGFR)-277(in)-277.8(cytokine)-270.3(release)-274.1(in)-277.8(response)-270.6(to)-278.9(FnIII)-272.8(domains.)-275.7(The)-271.2(specic)-277.8(roles)-271.1(of)-278.9(Src)-279.5(kinases)-270(an)]TJ 63.2542 0 TD (d)Tj -63.2542 -1.2448 TD [(EGFR)-291.2(in)-292.1(TLR4)-287.5(activation)-280.2(and)-292.5(signaling)-282.8(are)-288.8(not)-291(well)-287.9(understood.)-278.5(Possible)-288.4(interrelationsh)10.4(ips)-293.2(are)-288.8(depicted)-278.1(by)]TJ /F17 1 Tf 43.3261 0 TD [(dotted)-290.4(lines)]TJ /F16 1 Tf 4.7302 0 TD [(.)-294.1(The)-285.4(molecule)-286.7(labeled)-286.3(with)-288.9(a)]TJ /F17 1 Tf 12.2559 0 TD (question)Tj -60.3123 -1.2519 TD (mark)Tj /F16 1 Tf 2.2264 0 TD [(\(?\))-235.8(represents)-225.7(possible)-226.6(binding)-229.4(partners)-226.6(for)-237.5(FnIII)-230.1(domains)-223.4(that)-230.5(might)-237(particip)12.7(ate)-234.1(in)-235.2(TLR4)-230.6(activation)11.4(.)-237.2(T)0(o)-230.6(see)-231.7(this)-232.7(illustration)-231.7(in)-228(color,)-234.8(the)-234.1(reader)-221.9(i)]TJ 56.554 0 TD [(s)-238.4(referred)-226.8(to)]TJ -58.7804 -1.2519 TD [(the)-333.7(web)-323.5(version)-327.1(of)-335.8(this)-332.3(article)-323.7(at)-335.8(www.liebe)11.6(rtpub.com/w)12.9(ound)]TJ /F11 1 Tf 8.9663 0 0 8.9663 62.022 758.6644 Tm (316)Tj /F12 1 Tf 9.9626 0 0 9.9626 277.7952 758.6644 Tm [(VALEN)-11.3(TY)-336.3(ET)-336.3(AL.)]TJ ET 62.022 753.902 508.082 .51025 re f endstream endobj 29 0 obj <>stream Adobed         s!1AQa"q2B#R3b$r%C4Scs5D'6Tdt& EFVU(eufv7GWgw8HXhx)9IYiy*:JZjzm!1AQa"q2#BRbr3$4CS%cs5DT &6E'dtU7()󄔤euFVfvGWgw8HXhx9IYiy*:JZjz?N*S6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثba|ˡ^i|uda!LDfy ѫ:(1"<>Ɋ2<[%BiL-[ɞO!wū_\b%/H<)$cdF&EGfU"np=)3U6EI=fT y|L̵kx}XeY=Y.mȱ[p0F'Y;Fs<3k7V'ڟ7k拹Lzl$Hkzf\F0<ff˨Gpg i<oBh㖜ž?9HuhiN,HbkgCL2ɸf]vlU؅HS4α,h& nq[Ei^]-i5ooK! **vqb (-ڞ8#'P7JS w'aYg4H"O@m8ys<>^ԡ0ZI^uEQ,(5IDTI %HnDWHM>bex]2R!Y=9pN KY{yc&iitjp]UX yS! 7ÊyIqc1֛Ϝgʾbo+en M?/`Ѽ)9."t%Ϲg<j'2Drg@9>,<>b-"#&"GIJɿl,<ըT6HT|FP=BzY{ͼ?"j~6}'U/3iPNU :!ߓw(u};Ons(mW,2kvz햣vnPBi c[:Eb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ā銶&r{f GQk6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ثb͊6*ث*J!IqxdP8Qg H{DDB"(ĒMef]2~`yc@mnMƧ':]`"Jj 12yC~i,m[Zy( S6f)E-]_Ґ-֣2^,?5(z;#s3+YIgmݔH2֠ ot4hʖr3@u! 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[(ments)-290.5(for)-297.1(an)-291.9(MS)-292.3(degree)-294.7(in)-293.4(Biomedical)-293.3(Sciences)-291.7(at)]TJ 0 -1.1923 TD [(the)-240.7(Albany)-239.1(Medical)-243.8(Center)-239.5(\(AMC\))-240.7(and)-240.1(is)-238(currently)]TJ T* [(a)-401.9(student)-401.2(in)-401.8(the)-397.9(Physician)-405(Assistant)-396.7(Program)-400.6(at)]TJ ET .92 .92 .92 rg 251.036 541.587 298.885 178.129 re f 0 0 0 rg 251.036 718.696 298.885 1.0204 re f 251.036 541.587 298.885 1.0204 re f 251.036 541.587 1.0205 178.129 re f 548.957 541.587 .96381 178.129 re f BT /F2 1 Tf 10.959 0 0 10.959 257.0456 705.94 Tm [(KEY)-330.2(FINDINGS)]TJ /F9 1 Tf 9.9626 0 0 9.9626 267.0236 688.025 Tm ()Tj /F8 1 Tf 1.0015 0 TD [(Synergism)-298.4(in)-291(the)-287.5(TLR4)-292.3(signal)-11.8(ing)-287.9(pat)-8.6(hway)-291.4(activate)-11.2(d)-287.1(i)0(n)-291(skin)-289.2(brobl)-12(asts)-288.8(by)]TJ 0 -1.195 TD [(DAMPs)-520.9(\(FnEDA)-523.1(and)-525.5(FnIII-1c\))-523.3(wa)-8(s)-514.1(demons)-11.9(trated)-524.7(at)-518.2(the)-520.8(level)-521.4(of)-518.2(IKK)]TJ 0 -1.2007 TD [(phosphoryl)-15.3(ation)-339.5(and)-337.7(in)-336.5(the)-338.7(nuclear)-344(transloc)-11.2(ation)-339.5(of)-336.1(NF-)]TJ /F6 1 Tf 20.7819 0 TD (j)Tj /F8 1 Tf .552 0 TD (B.)Tj /F9 1 Tf -22.3354 -1.5023 TD ()Tj /F8 1 Tf 1.0015 0 TD [(Inhibitors)-355.3(of)-347.5(Src)-347.7(and)-349.1(EGFR)-346.7(kinases)-353.2(par)-8.6(tially)-349.9(blocked)-353.8(cytokine)-356.7(release)-349.7(in)]TJ 0 -1.2007 TD [(response)-390.1(to)-381.7(Fn)-380.1(DAMPs)-8.7(,)-379.5(suggestin)-12.7(g)-378.1(tha)-8.6(t)-379.1(these)-383(kinases)-387.3(are)-384.2(function)-11.7(ally)]TJ T* [(linked)-341.8(to)-336.1(TLR4)-337.9(activation.)]TJ /F9 1 Tf -1.0015 -1.4966 TD ()Tj /F8 1 Tf 1.0015 0 TD [(Synergism)-258.6(in)-245.5(the)-247.6(releas)-11.4(e)-247.2(o)0(f)-245.1(cyt)-7.4(okines)-254.5(from)-248.4(skin)-249.4(brobl)-12(asts)-249(was)-249(also)-250(seen)]TJ 0 -1.2007 TD [(between)-300.6(FnI)-8.4(II-1c)-294.7(and)-297.9(pat)-8.6(hogen-)-10.6(derived)-298.4(LPS)-301.2(but)-293.2(not)-298.8(betwe)-11(en)-295.3(FnEDA)-297.2(and)]TJ T* [(LPS,)-333.6(ind)-9(icating)-339.7(ligand)-342.4(spec)-9(icity)-342.2(to)-330.4(the)-338.7(syne)-9(rgistic)-340(respons)-11.9(e.)]TJ /F9 1 Tf -1.0015 -1.5023 TD ()Tj /F8 1 Tf 1.0015 0 TD [(Combinat)-12.5(ion)-435.8(of)-432.9(Fn)-437(DAMPs)-441.2(and)-434.4(LPS)-437.8(did)-441.5(not)-435.4(yield)-436(syne)-9(rgistic)-442.5(cytokine)]TJ 0 -1.195 TD [(release)-429.3(in)-427.6(monocyti)-13.1(c)-423.1(cells,)-428(indicating)-434(a)-423.7(cel)-8.4(l-type)-427.7(respons)-11.9(e)-423.7(t)0(o)-421.5(TLR4)-428.9(li-)]TJ 0 -1.2007 TD (gands.)Tj /F12 1 Tf -11.3868 21.1062 TD [(INDUCT)-11.7(ION)-336.9(OF)-334.3(IL-8)-337.1(BY)-336.1(FIBR)-8.8(ONECT)-9.3(IN)-332.6(TYPE)-343.1(III)-334.9(DOMAI)-9.9(NS)]TJ /F11 1 Tf 8.9663 0 0 8.9663 535.0676 757.8707 Tm (317)Tj ET 41.839 753.959 508.082 .51025 re f endstream endobj 33 0 obj <> endobj 34 0 obj <>/ProcSet[/PDF/Text]/ExtGState<>>> endobj 35 0 obj <>stream /GS1 gs BT /F5 1 Tf 10.4607 0 0 10.4607 62.022 712.3463 Tm 0 0 0 rg 0 Tc 0 Tw (AMC.)Tj /F18 1 Tf 2.9374 0 TD [(Christine)-267.9(M.)-268.3(Longo,)]TJ /F5 1 Tf 10.5195 0 TD [(received)-267.3(an)-270.2(MD)-271.8(from)]TJ -13.4569 -1.1923 TD [(Ross)-331.9(University,)-330.4(completed)-329.3(an)-329.8(internship)-331.4(in)-331.3(Gen-)]TJ 0 -1.1869 TD [(eral)-362.2(Surgery)-360.5(at)-358.3(AMC,)-360.1(and)-359.4(is)-362.7(currently)-359.2(a)-358.5(postdoc-)]TJ 0 -1.1923 TD [(toral)-210.3(fellow)-215.7(in)-212.1(the)-213.6(Department)-210.4(of)-207.6(Regenerative)-214.6(and)]TJ T* [(Cancer)-320.6(Cell)-320.4(Biology)-315.5(\(RCCB\))-320.7(at)-320.3(AMC.)]TJ /F18 1 Tf 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[(proteomic)-663.1(analysis)-667.3(distinguishes)-669.6(tissue)-668(repair)]TJ T* [(biomarke)9(r)-259.6(signatures)-255.3(in)-266.8(wound)-256.1(exudates)-256.4(obtained)]TJ T* [(from)-352.2(normal)-360.6(healing)-359.2(and)-359(chronic)-363.3(wounds.)-357.3(J)-356.8(Pro-)]TJ 0 -1.2448 TD -.0002 Tc [(teome)-329.8(Res)-325.6(2010;9:47584766.)]TJ -.9816 -1.8779 TD 0 Tc [(3.)-316.6(McCarty)-333(SM,)-343.3(Percival)-337.4(SL.)-341(Proteases)-338.1(and)-344.8(delayed)]TJ .9816 -1.2519 TD [(wound)-327.2(healing.)-333.3(Adv)-330.3(Wound)-331(Care)-323.8(\(New)-331.2(Rochelle\))]TJ 0 -1.2448 TD [(2013;2:43)8.4(8447.)]TJ -.9816 -1.8779 TD [(4.)-316.6(Guba)]TJ 2.5038 .0427 TD ()Tj .3628 -.0427 TD [(n)-385.2(B)0(,)-377.4(Vas)-382.3(K,)-384.5(Balog)-373.7(Z,)-387.7(et)-381.7(al.)-383.1(Abnormal)-371.7(regu-)]TJ -1.885 -1.2519 TD [(lation)-417.4(of)-417.2(bronectin)-420.9(production)-413.8(by)-419.9(broblasts)-418.9(in)]TJ 0 -1.2448 TD [(psoriasis.)-324.8(Br)-335.8(J)-328.3(Dermatol)-331.4(2016;174:53)6.2(3541.)]TJ -.9816 -1.8779 TD -.0201 Tc [(5)-20.1(.)-336.7(Beutler)-372.2(B)3.3(.)-371.1(T).1(L)-5(R)3.3(4)-369.8(a).1(s)-374.9(t)4.8(he)-369.8(mamm)5.7(alian)-369.8(endotoxin)-369.8(sen-)]TJ .9816 -1.2519 TD [(sor.)-293(Curr)-294(Top)-284.5(Microbiol)-285.9(Immu)7.1(nol)-293(2002;270:109120.)]TJ -.9816 -1.8708 TD 0 Tc [(6.)-316.6(Muro)-249.1(AF,)-260.5(Chauhan)-253.7(AK,)-259.3(Gajovic)-250.2(S,)-263.6(et)-253.6(al.)-262.2(Regulated)]TJ .9816 -1.2519 TD [(splicing)-371.9(of)-367.4(the)-375.7(bronectin)-371.1(EDA)-369.6(exon)-372.4(is)-378.6(essential)]TJ T* [(for)-435(proper)-438.4(skin)-435.8(wound)-441.1(healing)-437.5(and)-437.3(normal)-438.9(life-)]TJ T* -.0002 Tc [(span.)-325.3(J)-335.6(Cell)-331.5(Biol)-331.5(2003;162:149160.)]TJ -.9816 -1.8708 TD 0 Tc [(7.)-316.6(Ffrench-C)11.2(onstant)-380.6(C,)-384.5(van)-378.4(de)-379.3(Water)-378.4(L,)-378.6(Dvorak)-383.8(HF,)]TJ .9816 -1.2519 TD [(Hynes)-266.5(RO.)-273.6(Reappearance)-266.8(of)-275(an)-272.6(embryonic)-267.5(pattern)]TJ T* [(of)-239.4(bronectin)-236(splicing)-243.9(during)-238.2(wound)-241.9(healing)-238.3(in)-245.5(the)]TJ 0 -1.2448 TD [(adult)-329.5(rat.)-330.8(J)-335.4(Cell)-331.3(Biol)-331.3(1989;109:9)7.3(03914.)]TJ -.9816 -2.0059 TD [(8.)-316.6(Stanley)-461.7(CM,)-464.2(Wang)-457.9(Y,)-469.9(Pal)-467.1(S,)-462.8(et)-467(al.)-461.4(Fibronectin)]TJ .9816 -1.2448 TD [(fragment)10.7(ation)-386.4(is)-378.6(a)-378.1(feature)-377.9(of)-381.7(peridontal)-381.6(disease)]TJ 0 -1.2519 TD [(sites)-602.9(and)-608(diabetic)-602.6(foot)-606.8(and)-608(leg)-602.3(wounds)-603.8(and)]TJ T* [(modies)-336(cell)-339.6(behavior.)-340.5(J)-342.6(Peritonto)9.7(l)-343.9(2008;79:861)]TJ 0 -1.2448 TD (875.)Tj -.9816 -2.0059 TD [(9.)-316.6(Bhattacha)11.6(ryya)-429.7(S,)-420.1(Tamaki)-420.2(Z,)-423.3(Wang)-422.3(W,)-428.5(et)-424.3(al.)-418.7(Fi-)]TJ .9816 -1.2448 TD (bronectin)Tj 5.9774 0 0 5.3127 100.8567 166.2236 Tm (EDA)Tj 7.9702 0 0 7.9702 112.8755 162.7086 Tm [(promotes)-340.5(chronic)-342(cutaneous)-335.7(brosis)]TJ -4.9009 -1.2519 TD [(through)-581.8(toll-like)-587.5(receptor)-583.3(signaling.)-591(Sci)-582.8(Transl)]TJ T* [(Med)-330.9(2014;6:23)8.4(2ra50.)]TJ -1.4795 -1.9988 TD [(10.)-310.6(McFadden)-275.6(JP,)-275.1(Basketter)-273.3(DA,)-271.3(Dearman)-276.7(RJ,)-271.9(Kimber)]TJ 1.4226 -1.2519 TD [(IR.)-572(Extra)-563.2(domain)-571.6(A-positive)-564.2(bronectin-)9.5(positive)]TJ 0 -1.2448 TD [(feedback)-420(loops)-424.7(and)-423.1(their)-417.3(association)-417(with)-422.4(cuta-)]TJ 0 -1.2519 TD [(neous)-345.1(inamma)9(tory)-341.6(disease.)-342.1(Clin)-345.5(Dermatol)-338.5(2011;)]TJ T* (29:257265.)Tj -1.4226 -2.1268 TD [(11.)-310.6(Andrews)-310.2(JP,)-310.6(Marttala)-309.6(J,)-309.5(Macarak)-305.3(E,)-316.6(Rosenblo)9(om)]TJ 1.4226 -1.2448 TD [(J,)-366.4(Uitto)-354.9(J.)-366.4(Keloid)-362(pathogen)9.9(esis:)-367.1(potential)-359.1(role)-356.8(of)]TJ 0 -1.2519 TD [(cellular)-283.9(bronectin)-285.8(with)-280.1(the)-283.2(EDA)-284.2(domain.)-282.5(J)-285.7(Invest)]TJ T* [(Dermatol)-331.4(2015;135:19)6.2(211924.)]TJ 20.5783 58.1285 TD [(12.)-310.6(Gondokaryono)-440.4(SP,)-449.7(Ushio)-440.6(H,)-446.4(Niyonsaba)-445.1(F,)-442.6(et)-445.7(al.)]TJ 1.4226 -1.2519 TD [(The)-600.9(extra)-591.7(domain)-592.9(A)-600.3(o)0(f)-595(bronectin)-591.6(stimulates)]TJ T* [(murine)-367.7(mast)-369.2(cells)-367.1(via)-365.5(toll-like)-367(receptor)-362.8(4.)-373.5(J)-363.9(Leu-)]TJ T* [(kocyte)-330.3(Biol)-331.3(2007;82:6576)7.1(65.)]TJ -1.4226 -1.8708 TD [(13.)-310.6(Kelsh)-250.8(R,)-242.3(You)-249.1(R,)-249.4(Horzempa)-242.5(C,)-249.4(Zheng)-247.5(M,)-252.9(McKeown-)]TJ 1.4226 -1.2519 TD [(Longo)-437.5(PJ.)-438.7(Regulation)-432.9(of)-438.6(the)-432.6(innate)-437.3(immune)-438(re-)]TJ T* [(sponse)-244.6(by)-249.2(bronectin:)-245.6(synergism)-244.1(between)-239.4(the)-247.6(III-1)]TJ 0 -1.2448 TD [(and)-330.6(EDA)-334(domains.)-331.4(PLoS)-326.5(One)-332.3(2014;9:e102)6.1(974.)]TJ -1.4226 -1.8779 TD [(14.)-310.6(Zheng)-546.2(M,)-544.6(Jones)-544.2(DM,)-540.3(Horzempa)-541.2(C,)-548.1(Prasad)-540.6(A,)]TJ 1.4226 -1.2519 TD [(McKeown-Long)10.8(o)-349.7(PM.)-346.5(The)-344.8(rst)-341.6(type)-346.3(III)-341.8(domain)-344(of)]TJ 0 -1.2448 TD [(bronectin)-428(is)-435.5(associated)-428.7(with)-429.5(the)-432.6(expression)-428.7(of)]TJ 0 -1.2519 TD [(cytokines)-574.9(within)-568.3(the)-574.8(lung)-582.1(tumor)-566.8(microenviron-)]TJ T* [(ment.)-331(J)-335.4(Cancer)-331.2(2011;2:47)8.4(8483.)]TJ -1.4226 -1.8708 TD [(15.)-310.6(You)-434.1(R,)-427.2(Zheng)-432.4(M,)-430.7(McKeown-Lo)13.1(ngo)-437.3(PJ.)-431.6(The)-430.2(rst)]TJ 1.4226 -1.2519 TD [(type)-367.7(III)-370.3(repeat)-367.2(in)-373.5(bronect)9.5(in)-373.5(activates)-365.9(an)-372.1(inam-)]TJ T* [(matory)-231.6(pathway)-237.4(in)-231.3(dermal)-232.6(broblasts.)-236.5(J)-235.9(Biol)-231.7(Chem)]TJ T* -.0002 Tc 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[(human)-327.3(dermal)-332.2(broblasts)]TJ -2.9804 -1.2519 TD (BSA)Tj /F9 1 Tf 1.8779 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(bovine)-326.2(serum)-334.7(albumin)]TJ -3.3787 -1.2519 TD (CD14)Tj /F9 1 Tf 2.2762 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(cluster)-330.1(of)-331.9(differentiat)7.3(ion)-332(14)]TJ -3.5352 -1.2448 TD (cDNA)Tj /F9 1 Tf 2.4327 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(compleme)9.9(ntary)-335.6(deoxyribonu)9.2(cleic)]TJ .8251 -1.2519 TD (acid)Tj -4.588 -1.2519 TD (DAMP)Tj /F9 1 Tf 2.6603 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(damage-)9.5(associated)-329.1(molecular)]TJ .8251 -1.2448 TD (pattern)Tj -4.8156 -1.2519 TD (DMEM)Tj /F9 1 Tf 2.8879 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(Dulbeccos)-322.7(modied)-330.9(eagle)-334.2(medium)]TJ -3.1582 -1.2519 TD (ECM)Tj /F9 1 Tf 2.0557 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(extracellula)10(r)-337.8(matrix)]TJ -2.9804 -1.2519 TD (EDA)Tj /F9 1 Tf 1.8779 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(extra)-328.5(domain)-329.8(A)]TJ -3.3147 -1.2448 TD (EGFR)Tj /F9 1 Tf 2.2122 0 TD 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1.8281 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(horserad)9.5(ish)-337.1(peroxidase)]TJ -2.703 -1.2448 TD (I)Tj /F6 1 Tf .2205 0 TD (j)Tj /F8 1 Tf .5477 0 TD (B)Tj /F9 1 Tf .8322 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(inhibitor)-325(of)-339(kappa)-330.8(B)]TJ -2.6532 -1.3799 TD (IKK)Tj /F9 1 Tf 1.5507 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(inhibitor)-325(of)-339(kappa)-330.8(B)-332.2(kinase)]TJ -2.767 -1.3728 TD (IL-8)Tj /F9 1 Tf 1.6645 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(interleukin)-325.2(8)]TJ -3.2009 -1.3728 TD (IRAK)Tj /F9 1 Tf 2.0984 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(interleukin)9.1(-1)-331.9(receptor-associated)]TJ .8251 -1.2519 TD (kinases)Tj -3.4783 -1.3728 TD (KCl)Tj /F9 1 Tf 1.5507 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(potassium)-324.4(chloride)]TJ -2.767 -1.38 TD (LPS)Tj /F9 1 Tf 1.6645 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(lipopolysacch)10.6(aride)]TJ -3.2151 -1.3728 TD (MD2)Tj /F9 1 Tf 2.1126 0 TD ()Tj /F8 1 Tf 1.1025 0 TD [(messag)10(e)-335.4(digest)-328.6(algorithm)-332.2(2)]TJ ET 392.542 720.227 157.379 .51025 re f 392.542 399.742 157.379 .51022 re f 392.542 399.742 .51022 320.995 re f 549.411 399.742 .51019 320.995 re f BT 7.9702 0 0 7.9702 404.7873 707.5841 Tm (MMP2)Tj /F9 1 Tf 2.7883 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(matrix)-332.5(metallopept)7(idase-2)]TJ -3.7557 -1.2448 TD (mRNA)Tj /F9 1 Tf 2.6603 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(message)-332.6(ribonucleic)-327.9(acid)]TJ -3.144 -1.2519 TD (MyD)Tj /F9 1 Tf 2.0486 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(myeloid)-330.2(differentiation)-331.8(primary)]TJ .8322 -1.2519 TD [(response)-327.5(gene)]TJ -4.0403 -1.2448 TD (NaCl)Tj /F9 1 Tf 2.1126 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(sodium)-334.8(chloride)]TJ -2.8737 -1.2519 TD (NaF)Tj /F9 1 Tf 1.7783 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(sodium)-334.8(uoride)]TJ -3.7557 -1.2519 TD (NF-)Tj /F6 1 Tf 1.2733 0 TD (j)Tj /F8 1 Tf .5548 0 TD (B)Tj /F9 1 Tf .8322 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(nuclear)-329.8(factor)-332.1(kappa-light)-330.9(chain)]TJ .8322 -1.2519 TD [(enhancer)-329.5(of)-331.9(activated)-332.4(B)-332.2(cells)]TJ -3.3218 -1.2448 TD (NT)Tj /F9 1 Tf 1.3942 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(not)-333(treated)]TJ -3.6562 -1.2519 TD (PAMP)Tj /F9 1 Tf 2.5607 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(pathogen-asso)5.9(ciated)-335.7(molecular)]TJ .8322 -1.2519 TD (pattern)Tj -3.7059 -1.2519 TD (PBS)Tj /F9 1 Tf 1.7783 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(phosphate-buff)5.1(ered)-334.1(saline)]TJ -2.8737 -1.2448 TD (PCR)Tj /F9 1 Tf 1.7783 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(polymerase)-332.6(chain)-332.2(reaction)]TJ -3.3716 -1.2519 TD (p-IKK)Tj /F9 1 Tf 2.2762 0 TD ()Tj /F8 1 Tf 1.0954 0 TD -.0002 Tc [(phospho-inhibitor)-332.1(of)-332.1(kappa)-338.1(B)]TJ -3.5423 -1.2519 TD 0 Tc (PMSF)Tj /F9 1 Tf 2.4469 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(phenylmethane)-330.9(sulfonly)-330.3(uoride)]TJ -3.3218 -1.2448 TD (qPCR)Tj /F9 1 Tf 2.2264 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(quantitative)-334.5(polymera)9(se)]TJ .8322 -1.2519 TD [(chain)-332.2(reaction)]TJ -5.3775 -1.2519 TD (qRT-PCR)Tj /F9 1 Tf 3.4499 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(quantitative)-334.5(reverse)-328.8(transcription-)]TJ .8322 -1.2519 TD [(polymerase)-325.5(chain)-332.2(reaction)]TJ -3.9264 -1.3728 TD (RNA)Tj /F9 1 Tf 1.9988 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(ribonucleic)-335(acid)]TJ -3.3716 -1.3728 TD (RPMI)Tj /F9 1 Tf 2.2762 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(Roswell)-334.2(Park)-332.1(Memorial)]TJ .8322 -1.2519 TD [(Institute)-331.1(medium)]TJ -3.8126 -1.3728 TD (SDS)Tj /F9 1 Tf 1.885 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(sodium)-334.8(dodecyl)-325.3(sulfate)]TJ -3.8126 -1.3799 TD (S.E.M.)Tj /F9 1 Tf 2.7172 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(standard)-331.9(error)-332.9(of)-331.9(the)-333(mean)]TJ -3.3218 -1.3728 TD (TBST)Tj /F9 1 Tf 2.2264 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(tris-buffered)-327.2(saline)-334.7(Tween)-327.2(20)]TJ -3.5993 -1.3728 TD (THP-1)Tj /F9 1 Tf 2.5038 0 TD ()Tj /F8 1 Tf 1.0954 0 TD [(human)-334.4(leukemia)-331.4(monocytic)-329.5(cell)-332.5(line)]TJ -2.7599 -1.38 TD (TLR)Tj 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